monensin

VIVIAN F F WU (ffwu@unixg.ubc.ca)
Thu, 31 Aug 1995 08:59:37 -0700 (PDT)

Greetings to all!

I would like to hear from the practical experience of fellow cytometrists
who have worked with this nasty (very toxic) chemical.

Being insoluble in water means that we cannot dissolve the
lyophilized powder in PBS. It was suggested that monensin be dissolved in
DMSO. The concentration needed is in the range of uM. This was a
surprise to me as we use DMSO when we freeze down mononuclear cells,
although the concentration used in this context is considerably higher.

Question: Would dissolving Monensin in DMSO and adding 1-5 uM of it into
mononuclear cell cultures for < 24 hours affect the integrity of the cell
surface antigens?

Question: At what strength does Monensin ceases to become toxic?

Thanks in advance.

Vivian Wu
Immunology Lab.
Vancouver Hospital & HSC
B.C. Canada

e-mail: ffwu@unixg.ubc.ca

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