Re: Lymphocyte Clusters
Scott I. Simon (ssimon@bcm.tmc.edu)
Mon, 28 Aug 1995 13:09:57 -0500
As was already stated you could rely on Fsc-SSC in a linear mode
biparameter histogram and see a shift mostly in SSC, but diagonally as
well. A second approach is to label with a bright CD like CD45 and on FL1
or FL2 depending on the conjugate you should in a linear mode see
integral multiples of the singlet fluorescence. good luck.
Scott I Simon.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
