Lymphocyte Clusters

Dennis_Young@CIS.ucsd.edu
Fri, 25 Aug 1995 16:13:00 -0700

Using the DDM (Doublet Discrimination Module) can help, but
cells are not perfect little billard balls, so it won't
*EVER* be 100% accurate. The other problem is that conjugates
are rare, even when you try to make them!
The simplest way is to look at scatter signals. Cell clusters
will have higher Forward and Side scatter signal intensity.
See the recent postings on doublet disc in DNA analysis.

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* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
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Our lab uses a BD FACScan and performs three-color analysis on
human periphal blood lymphocytes. Does anyone know of the best
way to identify lymphocyte conjugates (clusters of two or more cells)?

Tim Patton
University of Pittsburgh

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