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I have a proplem with BD's CellQuest on a Macintosh Quadra with
16MB RAM:
When creating more than 6 mulitcolor Dotplots I get a message
saying that there is not enough memory anymore.
Is there any solution besides buying another 16MB of RAM and
switching memory swaping to on?
Any help welcomed!
Martin Wimmer
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Martin,
We have installed 40 meg of interleaved RAM on a Quadra 800. We have
allocated 16384K to the CellQuest program. Whenever we acquire files greater
than 250,000 events, as we do for rare event analysis or in setup mode for
sorts, we have a complete system crash and lockup when we try to print. The
problem is exacerbated by the number of plots that are open at any given time
and the length of time the program hs been running. All this has made me
suspect that the current version of CellQuest (1.2) may have a significant
"memory leak" That is, the program continues to exhauast greater and greater
memory space as it runs until there is not enough RAM left to run the program
and the print monitor/spooler at the same time. Unfortunately, the only fix for
this is to find the leak. This is something that the programmers at BDIS will
have to do.
Remember, with Lysys II you were able to sort millions of cells in setup
mode and still were able to print without any problems at all. (Also, you were
able to sort with a region and not a gate set.)
The best course of action is to contact your BDIS representative and
pressure them for a fix.
In the interim, allocate as much system memory to the program as possible
by clicking once on the CellQuest icon, and pressing Apple (correction) I. In
the lower
left of the info window that pops up you will be able to adjust the memory
allocated to the program. It is best to make the minimum and maximum values the
same. Also, when sorting, purge the memory by doing a pause and a restart
before trying to print a document. Obviously this will limit the number of
events you will be able to print out, but a total system lockup during a sort,
especially with an unsaved document, is, as you well know, calamitous.
Patience is a virtue, but how often do you feel virtuous?
Best Regards,
Mark
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Mark G. Edinger
Immunotyping Laboratory
Cleveland Clinic Foundation
Cleveland, Ohio
edingem@cesmtp.ccf.org
edingem@ccsmtp.ccf.org
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