Bovine sperm sorting

PAUL@flowcyt.cyto.purdue.edu
Fri, 25 Aug 1995 09:31:35

Roger Burger asked about sorting bovine sperm. We have a publication
on this - it was done by magnetic beads and confirmed by flow
cytometry sorting and analysis. The details are in the papers below.

Paul

Jones, P.P., Peter, A.T. and Robinson, J.Paul - Monoclonal antibody
12/49 separation of X and Y chromosome-bearing bovine spermatozoa
using immunomagnetic beads and flow cytometry. Proc.Conf.Res.Workers
Animal Dis. 72:30-Abstract 173, 1991.(Abstract)

Peter, A.T., Jones, P.P. and Robinson, J.Paul - Fractionation of
bovine spermatozoa for sex selection: a rapid immunomagnetic
technique to remove spermatozoa that contain the H-Y antigen.
Theriogenology 40:1177-1185, 1993.

J.Paul Robinson, Purdue University Cytometry Labs
robinson@flowcyt.cyto.purdue.edu PH:317-494 6449 FAX:317-494 0517
web http://www.cyto.purdue.edu


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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu