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Our question has to do with doublet correction. When we observe the
raw data in (dual parameter) Peak versus Integral fluorescence scatter plots,
we sometimes observe a fair percentage of so-called doublets, i.e., cells that
have 4N amounts of integral fluorescence with 2N amounts of "peak"
fluorescence.
Our question is this: what are the pros and cons of excluding these cells
from our analysis using a gating approach versus doublet correction using
software routines? I know that this subject has been approached in other
forums, but I'd like to hear current thoughts on the matter.
Phil Marder
Lilly Research Labs
From: MARDER PHILIP (MCVAX0::MARDER)
To: cyto-inbox
cc: MARDER PHILIP (MCVAX0::MARDER)
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