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When labeling leukocyte cell surface antigens with the appropriate
conjugated antibody, is it best to fix (with paraformaldehyde) the cell
suspension:
a) before antibody labeling?
b) after antibody labeling?
c) what concentration of paraformaldehyde (1% final concentration?)
and is it dependent upon the
d) cell type?
e) antigen to which the antibody is specific for?
f) monoclonal vs. polyclonal antibody?
If that wasn't enough for you, add in the variable of labeling intracellular
antigens into the question of timing of fixation.
If there is a review of this in the literature, could you point me to it
please.
Thanks for your time and forthcoming answers.
Dan J. Smith
San Diego CA
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