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I am in charge of a histology/ cytometry laboratory in Gaithersburg, MD. We
are doing immunoperoxidase/alk. phos. staining for various antigens in tissue,
cytospins, or cells cultured on coverslips or slides ( cell preps are handled
in this way when flow cytometry cannot be done) . I am looking for a good
image analysis system that will enable me to quantitate positive-staining
subpopulations in cell preps , or determine the proportion of positive-
staining cells in a defined area in tissue (i.e. PCNA-positive cells in
bronchial epithelium). Automated stage movement would be needed and slide
autoloading would be a welcome capability as well. Since we have also been
obtaining good multi-level staining for some antigens, it might be useful to
have some densitometry capabilities in the software, although this may be
considered icing on the cake or a much more sophisticated function that may
not be possible with one software package. Color discrimination is preferred
over gray-scale discrimination. To complicate matters even more, I would also
like to be able to use the same hardware to handle images from a conventional
mercury lamp fluorescence microscope, although at this time I am placing
priority on light microscopy, since that is where our quantitation needs are
the most immediate. I have a Nikon Optiphot with Nomarsky capabilities that
I use for light microscopy, and a Nikon Labophot which I use for my
fluorescence work. Is there anyone out there who can direct me to a few good
contacts so that I can put together a system that will work for me? Thank you
for your help!
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James L. Weaver Email: WEAVER@FDACD.CDER.FDA.GOV
Molecular Pharmacology Phone: 301-594-5879
Division of Research & Testing FAX: 301-594-3037
ORR, CDER, Food & Drug Admin. Voice: 'Hey you'
Laurel, MD
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