RE: surface staining macrophages
Edberg, Jeffrey Ph.D. (EdbergJ@hss.edu)
Mon, 14 Aug 95 16:15:00 PDT
Also keep in mind that the low affinity Fc receptor on mouse
macrophages is a "receptor" for PE (see an artical by J.-P. Kinet in J.
Immunol. Methods a few years back). This receptor can be blocked with the
rat mAb 2.4G2 which binds to both mouse FcRII and FcRIII. There are no mAb
available to the murine high affinity FcR (FcRI) but murine IgG2a binds
particularly well to this receptor. This binding can be a problem with
IgG2a mAb.
Jeff Edberg
Cornell University Medical Center
edbergj@hss.edu
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From: owner-cyto-sendout
To: Cytometry Mailing List
Subject: surface staining macrophages
Date: Monday, July 24, 1995 8:49PM
Hello. I'd like to look at expression of a surface protein on mouse
macrophage-
like cell lines by flow cytometry. I'm worried about decorating them with
our
mouse mAb, which could be bound by FcR's on these cells. Two options I
thought
of were 1) absorb mouse myeloma proteins to the FcR's, and FITC-label by
mAb;
2) cleave the mAb into Fab or F(ab')2 fragments which could be
FITC-conjugated.
What have other people done in these circumstances? What works and what
doesn't?
Thanks in advance,
Brett Lindenbach
Program in Immunology
Washington University - St Louis
brett@borcim.wustl.edu
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