Re: sorting on the slides
Kai Toellner (TOELLNKM@novell1.bham.ac.uk)
Fri, 11 Aug 1995 11:47:06 BST
Fatima Abbasi wrote:
>
> Sometimes I need to sort on the slide using Factar plus cell sorter
(BD).
> I put my slide on the sort tube holder assembly, which is slightly V
> shaped. This causes sorted cells to "bleed" towards the center. Is
there
> any assembly in the market which I am not aware of, specially to hold
> slides to sort on to.
> Any comments???
>
Use the ACDU unit for the FACStar plus from BD. With this, you can sort
into 96 well cell culture plates or terasakiplates. With a special slide
holder, which comes with the kit, you can also sort on microscopical
slides. It is even possible to move the slide during sorting to sort
different cells onto different spots on the slide. I have a photograph,
where I sorted single beads into arrays of 4 times 4 drops, with a
distance of the drops of 0.3 mm from each other. So it works quite
accurat.
If you just want to sort one batch of cells on a slide, you can put your
slide on top of the plexiglass box beneath the sorting chamber. Between
this box and the front door to the sorting chamber is a small gap.
Microscope slides just fit through the gap when the front door is closed.
When you press the slide onto the top of the plexiglass box it lies
perfectly plain. Maybe, you can even fix it there with adhesive tape, if
you have a long sort.
-----------------------------------------------------------
Kai-Michael Toellner
Department of Immunology
The Medical School Tel.: (44) 121 414 6970
University of Birmingham FAX.: (44) 121 414 3599
Birmingham B15 2TT, UK E-mail: K.M.Toellner@bham.ac.uk
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