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DEBORAH SHUEY GROVE (dsg4@genesis.ait.psu.edu)
Thu, 10 Aug 1995 08:34:28 -0500 (EST)
We are setting up studies to look at surface antigens on human PBL. We have
experience with rat splenocytes and lymph node cells and would like advice
on working with human PBL.
1) We are considering staining whole blood vs. separated.comments?
Population differences?
2) We would like to screen a panel of antibodies in as few samples as
possible by doing several markers in a single group. We can use FITC, PE,
and PECy5. Are there some combinations of markers that are best to use? For
example, TCRalphabeta, CD4, and CD8 may be good to run together to determine
what percentages of T are CD4 and CD8 and if any are double-labelled. Also,
NK with a T cell marker as a negative control?
3)Any advice on the best controls for human PBL? LCA?
4) Any advice on adhesion markers? We expect changes in circulating
populations.
5) Data analysis? Are there published examples of histograms that we can ue
for comparison?
Thanks in advance.
Deb Grove
Dept BMB
Penn State
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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EMAIL robinson@flowcyt.cyto.purdue.edu
