Re: Sorting bacteria
/G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Fri, 4 Aug 1995 13:58:00 -0400
Hello Ray
We routinely sort bacteria onto agar plates using an Epics
Elite with an autoclone attached and get >98% recovery
sorting for viable bacteria. Only that we use only 15mW
laserpower, dulbeccos buffered saline (made up from tablets
from Oxoid / Unipath, UK) and adjust the system with 0.66um
Beads.
Instead of sorting 1000 in a liquid you will be much better
of putting them right into single colonies (approx. 60 per
agar plate) as that is not so time-consuming (and usually
microbiologists can not handle 1000 clones anyhow). This
might also allow you to run a slower acquisition rate thus
reducing the coincidence problems at that low frequency.
It may be worthwhile checking the metabolic state of the
organisms whilst sorting depending what was done to them
prior to sorting and to make sure what you sort is a bug and
not any sort of schlunz.
Gerhard
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