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> Are there already shareware programms on the scene?
A comprehensive, international catalog of free flow cytometry software is
available via World Wide Web (using a Web browser such as Mosaic, Netscape,
or Lynx) at http://www.bio.umass.edu/mcbfacs/flowcat.html.
WinMDI (for PC Windows) is the preeminant free general purpose flow
cytometry program. It is quite excellent and does almost everything you
might want except DNA cell cycle analysis (and its capabilities for time
kinetics are limited). It does multiple colored gates and 3D plots. Get
it from http://facs.scripps.edu, or by anonymous ftp, also from
facs.scripps.edu in /pub/pc.
MFI (for DOS) is available by anonymous ftp from flowcyt.bio.umass.edu in
/pub/flowcyt/mfi. It is especially good for processing large numbers of
files quickly, for time kinetics (including calculating a ratio and drawing
the line graph), and for spreadsheet-ready ASCII conversions. It has a
detailed tutorial with sample data. MFI does neither quadrant analysis
nor DNA cell-cycle analysis.
More details are available in the catalog on these and other free programs.
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
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