Re: FDG staining for b-gal

S.P. Monard (spm1007@cus.cam.ac.uk)
Fri, 4 Aug 1995 11:59:32 +0100 (BST)

We have been using the FluoReporter LACZ kit from molecular probes part
number F1930 (50 test kit). It works well, the positive cells are a good
log up from untransfected cells. You need to keep the sample cool while
sorting otherwise the untransfected cells increase their fluorescence
over time. We just follow the intructions. The cells we have been using
are human fibroblasts and endothelial cells though.

Good luck

Simon Monard
Cambridge
UK

Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu