fluorescence intensity question

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1) Cell type A lacks receptor X expression and autofluoresces in channel 200.
Type A cells stained with FITC-conjugated anti-X mAb also fluoresces in
channel 200.
2) Cell type B has 10,000 X molecules on its cell surface and type B cells
stained with FITC-conjugated anti-X mAb fluoresces in channel 400.
( linear scale )

Inquiry: If cell type C has 5,000 X molecules on its cell surface and is
stained with FITC-conjugated anti-X mAb, in which channel will it
fluoresce?

Thanks for your comments,
Jim Tarara

James E. Tarara
Flow Cytometry/Optical Morphology Research Core Facility
Mayo Clinic/Foundation
Rochester, MN 55905
(507) 284-4241
Tarara.james@mayo.edu

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu