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Eric Miller wanted some more info about the question I posted about apoptosis
analysis with Modfit.
The samples we are working with are those of human CD34+ cells kept in culture
for different time points. If a sample contains apoptotic cells, it is easy
to "find" and quantitate the percentage of apoptic cells. This is ofcourse
true if you have apoptotic cells in your test sample. If you don't, and this
may be the case with your samples, whether you use a FACScan or something else
will be irrelevant. Indeed some of our samples do not contain, or contain
few, apoptotic cells and the analysis reveals that. The problem I decribed
earlier is encountered in few cases only especially in samples were the degree
of apoptosis is high.
--------------------------------------
Date: 8/2/95 12:07 PM
To: cyto-inbox
From: Eric Miller
On Tue, 1 Aug 1995, Edward Srour wrote:
> We have been trying to determine percentage of apoptotic cells using PI
> staining and analysis of DNA histograms with Modfit LT. The program works
> very nicely, but occasionally we get a histogram in which a substantial
number
> of cells appearing in the G0/G1 and the S peaks are considered by Modfit as
> apoptotic cells. Verity does not provide any "explanation" or suggestions
> regarding how to get around that. Did anyone out there encounter this same
> problem and how was it resolved? Thanx for any info.
>
> Edward F. Srour, Ph.D.
>
>
Could you post a little more info on this? I have not managed to successfully
identify apoptosis on a DNA histogram on a Facscan. The samples are FNAs of
xenografted human breast tumour cell lines.
Eric Miller, ICRF Medical Oncology Unit, Edinburgh UK
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Date: Wed, 2 Aug 1995 11:44:07 +0100 (BST)
From: Eric Miller <miller@icrf.icnet.uk>
Subject: Re: Apoptosis with Modfit
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