Higher sensitivity
Mario Roederer (ROEDERER@Darwin.Stanford.EDU)
Wed, 02 Aug 1995 09:18:47 -0700 (PDT)
We have successfully used a system devised by Peter Lansdorp up in
Vancouver (at the Terry Fox Cancer Center). He developed an anti-PE
antibody... the basic procedure is to stain cells with and
PE-conjugated antibody to what your interested in. Then, do successive
iterations of staining with biotinylated anti-PE (from Peter), followed
by PE-conjugated avidin. (Several washes between each step!). We
found no significant increase in background staining, and after 3
rounds we had a 5-10 fold amplification of signal.
It's a pretty cute system. For information, please contact him directly.
mr
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
