Apoptosis with Modfit

Edward Srour (edward_srour@iucc.iupui.edu)
Wed, 02 Aug 1995 08:48:35 -0500

We have been trying to determine percentage of apoptotic cells using PI
staining and analysis of DNA histograms with Modfit LT. The program works
very nicely, but occasionally we get a histogram in which a substantial number
of cells appearing in the G0/G1 and the S peaks are considered by Modfit as
apoptotic cells. Verity does not provide any "explanation" or suggestions
regarding how to get around that. Did anyone out there encounter this same
problem and how was it resolved? Thanx for any info.

Edward F. Srour, Ph.D.

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