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My self and Dr. Gary Michels have been investigating growth factor
receptors on osteosarcoma cells using flow cytometry. We have been using
a conventional monoclonal mouse-anti receptor antibody and FITC goat-anti
mouse secondary antibody. Since the receptor density per cell is less
than five hundred, there is only a small shift in fluorescence between the
positive and negative control samples.
I have heard there are methods using a triple antibody biotin avidin
combination, but that is all. Can anyone help?
Steve Vokal
Orthopedic Research
Creighton University
Omaha, Nebraska
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