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Course Organisers: Dr M G Ormerod, Reigate, Dr M Lowdell, Royal Free Hospital Medical School
BASIC COURSE: Monday 18 - Wednesday 20 September 1995
ADVANCED COURSE: Wednesday 20 - Friday 22 September 1995
COMBINED COURSE: Monday 18 - Friday 22 September 1995
The two courses - basic and advanced - will run sequentially to give the student the choice of attending
either or both of the courses. It is anticipated that, with the generous co-operation of the manufacturers,
Becton-Dickinson, Coulter and Ortho, there will be three bench top cytometers for use by the students.
Basic Course
The basic course will assume little prior experience and take the student through the most important
applications. Although it will fill the needs of someone working in a research environment, it will have a slight
clinical bias.
Topics covered will include: The basics of flow cytometry; fluorescence, fluorochromes and optical filters;
data analysis, including handling multiparametric data; detection of cell surface antigens; DNA analysis
including cell cycle analysis; antigens associated with cell proliferation.
During the last afternoon, there will be a choice of modules. Module 1: Immunophenotyping (including
immunophenotyping of leukaemias and lymphomas, quality control and flow cytometry in a clinical
environment); Module 2: Measurement of tumour cell kinetics (including measurement of DNA in tumours
and the cell cycle analysis of tumours using BrdU).
Practicals will include: three colour immunofluorescent analysis of human lymphocytes, measurement of a
DNA histogram, measurement of a nuclear antigen, immunophenotyping leukaemias, estimation of MESF,
measurement of a DNA histogram from paraffin-embedded tissue and cell cycle analysis of tumours using
the BrdU-anti-BrdU technique.
Advanced Course
The advanced students will join the last day of the basic course. Topics covered will include: cell cycle
analysis using BrdU/anti-BrdU and using BrdU/Hoechst-propidium iodide; chromosome analysis and sorting;
applications in cell and molecular biology (including measurement of intracellular calcium and pH); apoptosis;
multi-drug resistance in tumours; and the measurement of enzyme kinetics.
Lectures will be supported by practical sessions which will include cell cycle analysis of tumours using the
BrdU-anti-BrdU technique; preparation and analysis of chromosomes; intracellular enzyme kinetics using
the BrdU-Hoechst/PI method and and measuring apoptotic cells using the terminal deoxynucleotidyl
transferase assay.
Application forms are available from: The Administrator, The Royal Microscopical Society, 37/38 St
Clements, Oxford OX4 1AJ, England. Telephone: (01865) 248768 Fax: (01865) 791237
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