surface staining macrophages
Calman Prussin (Calman_Prussin@d10.niaid.pc.niaid.nih.gov)
Thu, 27 Jul 95 17:09:30 EST
In the mouse system one typically uses the clone 2.4G2, which is a mAb directed
against a shared epitope of the Fc-gamma RII and RIII. This method of blocking
is vastly more effective than using an excess of IgG or serum. The 2.4G2 cell
line is available from ATCC or you can buy it from Pharmingen as "Fc block" or
some such thing.
surfs up, gotta'go.
cp
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Brett Lindenbach wrote:
>Hello. I'd like to look at expression of a surface protein on mouse
>macrophage-
>like cell lines by flow cytometry. I'm worried about decorating them with our
>mouse mAb, which could be bound by FcR's on these cells. Two options I thought
>of were 1) absorb mouse myeloma proteins to the FcR's, and FITC-label by mAb;
>2) cleave the mAb into Fab or F(ab')2 fragments which could be
>FITC-conjugated.
>What have other people done in these circumstances? What works and what
doesn't?
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
