RE: DAPI
kukuruga%kasle1.dnet@rocdec.roc.wayne.edu
Thu, 27 Jul 95 17:23:49 -0400
No...DAPI is not a vital stain. Some while back someone told me it was
and we tried it (compared it with H33342). No DNA histogram. I haven't
looked at my Mol. Probes guide yet, but I think it reads as DAPI being
perhaps a "viability" probe -- like PI -- and only dead cells will label.
Whatever....bottom line...you have to fix your cells. If you do, however,
you will find DAPI out-performs H33342 significantly.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
