surface staining macrophages
brett (brett@borcim.wustl.edu)
Mon, 24 Jul 1995 20:49:04 -0600
Hello. I'd like to look at expression of a surface protein on mouse macrophage-
like cell lines by flow cytometry. I'm worried about decorating them with our
mouse mAb, which could be bound by FcR's on these cells. Two options I thought
of were 1) absorb mouse myeloma proteins to the FcR's, and FITC-label by mAb;
2) cleave the mAb into Fab or F(ab')2 fragments which could be FITC-conjugated.
What have other people done in these circumstances? What works and what doesn't?
Thanks in advance,
Brett Lindenbach
Program in Immunology
Washington University - St Louis
brett@borcim.wustl.edu
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
