Re[2]: Antibromodeoxyuridine MoAb

Ric_Thorpe_at_APPLIC1@ccmail.llu.edu
Mon, 17 Jul 95 09:56:48 PST

Robin,

Now that you have piqued our interest in the BU-1 clone in particular,
(assuming you eventually found it), can you tell us where to get the
stuff. It sounds interesting.

Thanks!

Ric Thorpe
Loma Linda Immunology Center
Loma Linda, CA USA

Email: Ric_Thorpe_at_APPLIC1@ccmail.llu.edu

______________________________ Reply Separator _________________________________
Subject: Re: Antibromodeoxyuridine MoAb
Author: Robin.Allen@WPO4.CL.HealthWaikato.HWL.synet.net.nz at InternetMail
Date: 7/14/95 5:39 PM

Many thanks to all who replied to my request for a source of the BU-1
clone.

In reply to those who asked why the BU-1 clone in particular.

The commonly used antibromodeoxyuridine antibodies react only with
single stranded DNA which requires pretreatment with HCl for cleavage.
The BU-1 clone avoids denaturation of the DNA due to DNase activity.
This leaves cell morphology as well as surface or cytoplasmic markers
intact.


Robin Allen
Haematology Dept
Waikato Hospital
Hamilton
New Zealand

E-mail Robin.Allen@wpo4.cl.healthwaikato.hwl.synet.net.nz
Fax 64 7 8398759

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu