Saturation
Edgar Milford (emilford@bustoff.bwh.harvard.edu)
Thu, 20 Jul 1995 07:47:00 -0500
In response to TIA's query about range of antibody "concentrations" for
saturation, this depends upon a number of variables including
Number of molecules of target on the cell
Number of cells
Number of epitopes per molecule
Affinity of the antibody
Temperature of the incubation
Length of time of the incubation
Sometimes even wash conditions if a weak antibody.
Amount of nonspecific binding.
A book or article dealing with antibody kinetics would help you
conceptualize these things. The bottom line is you really need to deal
empirically with the issue in your particular system.
Edgar L. Milford, M.D.
Brigham and Women's Hospital
Tissue Typing Laboratory
75 Francis Street, Boston, MA, 02115
Tel: 617 - 732-5872
Fax: 617 - 566-6176
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
