Quantitative Image Analysis
Clint E. Young (clintey@unixg.ubc.ca)
Sat, 1 Jul 1995 15:59:25 -0700 (PDT)
To fellow collegues
There does not seem to be a forum (mailing list, discussion group, etc..)
singularly devoted to the subject of quantitative image analysis (Q-IA).
I would, therefore, like to create a mailing list of sorts.
First, let me explain what my lab is doing.
In my lab, quantitative image analysis (Q-IA) is used in combination with
immunohistochemistry and Western Blotting. The purpose of Q-IA, in the
context of our lab work, is to capture images of immunostained tissue and
immunoblots in order to determine the amount of staining and, therefore,
the amount of antigen present.
Q-IA of immunostained tissue can also be used to determine:
A. MACROSCOPIC FIELD
1. Number of cells stained
2. Size of cells
3. Distribution of cells
B. MICROSCOPIC FIELD
1. Amount of antigen present
2. Distribution of antigen
3. Colocalization of different antigens (confocal)
Q-IA of immunoblots, meanwhile, is used primarily for B.1-2.
To perform this type of analysis one needs peripherals (digital camera
and/or flatbed scanner) hardware (PC or Macintosh computer), and software
(NIH Image, Adobe Photoshop, etc...).
Obviously, Q-IA can be performed using many different setups depending on
the combination of peripherals-hardware-software used.
I would like, therefore, to make a list of those interested in this topic
and will circulate this list among the participants.
Please include the following:
1. E-Mail address
2. Computer
3. Image Analysis Software
4. Type of analysis you are CURRENTLY ABLE to perform
5. Question/Comment
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The following is an example of what I would send:
clintey@unixg.ubc.ca
Macintosh
NIH Image
IHC: A(1,2,3) + B(1,2,3)
WB: B(1,2)
?: I am taking images of Western Blots using reflective light; I've tried
making the WB transparent but the process causes the staining to run or
streak. Does anyone know which method--reflective or transmitted
light--works best for quantifying Western Blots?
----------
Please E-Mail me directly since this message will be sent to various
mailing lists.
I will send participants the compiled list on Monday July 3.
Looking forward to your reply.
Clint Young
Department of Psychiatry
University of British Columbia
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