question on linearity of flow channels

Peter D Briggs (pbriggs@uoguelph.ca)
Fri, 30 Jun 1995 15:06:47 -0400 (EDT)

Hi, just a small question that I hope someone can answer:

I'm doing research on polyploidy in several plant species and
have been using flow to determine ploidy level. In order to quantify and
compare, I have been running CRBC's as an external standard. After every
five samples run, I run the CRBC's and adjust the voltage to get the CRBC
peak at approximately channel 100 on the PMT3 screen. My question is,
what sort of a relationship is there between voltage and channel
shifting? If you adjust the voltage to bring the CRBC peak up 10 channels,
is it a linear relationship with all channels being shifted 10 as well?
Or is there some non-linear relationship?
Some of our samples have been giving us consistently weird
results, and if a non-linear shift is possible, that would explain what
we're seeing.
Thanks in advance for your input...

Peter Briggs
pbriggs@uoguelph.ca

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