CD34+: methylcellulose colony assay vs. FACS analysis
Penney Koeppen (Penney_Koeppen@hgsi.com)
Fri, 30 Jun 95 11:20:52 EDT
Has anyone used FACS analysis of liquid cultures of CD34+ cells from cord blood
to reproduce data obtained from parallel methylcellulose colony assays? eg:
Would the number of BFU-E's in methylcellulose correlate with % glycophorin A-
positive cells in a parallel liquid culture; and would the number of CFU-meg's
correlate with % CD41a-positive cells?
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu