Re: Suspension Preparation from Solid Tumours
PAUL@flowcyt.cyto.purdue.edu
Tue, 20 Jun 1995 17:50:47
Eric Miller noted:
> I read recently in the lab press that Dako are marketing a machine which
> they claim will produce a suspension of cells from solid tumours. Has
> anyone any experience of using this machine? I would be particularly
> interested in efficiency of disaggregation, running costs etc. in
> relation to breast tumours.
> Eric Miller, ICRF Medical Oncology, Edinburgh UK
Phoenix flow systems sells a system for this purpose. The unit costs
under $2000 and the individual "mincers" cost about $4.50 ea - so
they are not cheap. I have one coming in this month to use it for a
study where we will use it for liver and spleen on 100 animals. I
hope that it saves us a bunch of time and give more uniformity of
preparation. We just could not do all the animals in one day and run
all the samples without this device. So we'll see how it goes...I
have no experience using it with tumours or even tumors!
Paul
J.Paul Robinson, Purdue University Cytometry Labs
robinson@flowcyt.cyto.purdue.edu PH:317-494 6449 FAX:317-494 0517
web http://www.cyto.purdue.edu
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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