Tricky AO population
system@flocyt.int-med.uiowa.edu
Fri, 16 Jun 1995 14:43:18 EDT
We have an investigator studying apoptosis using acridine
orange (AO) as one of the assays. We are not sure how to
categorize one of the populations that we see in a mouse
lymphocyte model. This particular sample is not proliferating
but is apoptotic and we see three populations => the usual tight
G0 population, a tight apoptotic population that has the same
RNA content but less DNA content than G0, and a smeared
population that has both less RNA and DNA than the G0 or
apoptotic population so that it tails off towards the baselines
both in RNA and DNA content in a linear relation, i.e.; it slants.
The latter population is the one in question and we're scratching
our heads wondering what to do with it. We have gated out the
obvious debris that sits way down in the left corner of a
Forward Scatter vs. Orthogonal Scatter plot. In the old days
before apoptosis became a fad, we would have just gated out
this weird population. Any ideas?
Justin Fishbaugh
University of Iowa
Flow Cytometry Facility
internet: justin-fishbaugh@uiowa.edu
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