Bleaching TransFluoSpheres

Graham Vesey (GVESEY@rna.bio.mq.edu.au)
Fri, 16 Jun 1995 13:39:35 GMT+1000

I'm using high numbers of fluorescent beads (TransfluoSpheres, Molecular
Probes) to label cells. I'm having problems with carry over of beads from
sample to sample.

Our normal procedure for stopping carry over is to run 10% sodium
hypochlorite between samples. This removals the fluorescence from
everything we've tried - but it doesn't work with TransFlouSpheres.

I'm analysing on an Elite, an XL and a Facscan.

Any suggestions as to how to stop these beads fluorescing.
_______________________________________________
Graham Vesey
Australian Environmental Flow Cytometry Group
School of Biological Sciences, .-.--:_:\
Macquarie University, _/ \
Sydney, : AEFCG |
Australia NSW 2109. \_ /
Tel- 612 850 8150 '-''''\__/
Fax- 612 850 8174 V
_______________________________________________

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