PerCP chemistry
Frederic Preffer (preffer@HELIX.MGH.HARVARD.EDU)
Tue, 13 Jun 1995 10:34:00 -0500 (EST)
Can someone please outline the physical attributes of PerCP, with respect
to that dyes requirements for excitation time, laser power requirements,
etc. How do these compare/contrast with the requirements of something like
FITC, PE, or APC?
For example, I see that PerCP works well when exited within the cuvette
of a FACScan, but not in a stream-in-air sorter, ostensibly due to the
longer time and lower laser-power requirements of the dye. Can someone give
me the real FACS? thanks.
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Frederic I. Preffer
preffer@helix.mgh.harvard.edu
Department of Pathology- Cox5
100 Blossom St
Massachusetts General Hospital
Boston MA 02114
(617) 726-7481 {fax x2365}
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