Re: CD59
Brian Smith (smith@beaker.med.yale.edu)
Wed, 7 Jun 95 11:43:35 -0400
We actually do PNH screening as a routinely offered test at Yale-New Haven
Hospital in New Haven, CT. We measure LFA-3 (CD58), DAF (CD55), and MIRL
(CD59) on both the red cells and on the leukocytes, the latter subdivided
into lymphs, monos, and polies. It is especially useful for patients who
have been transfused since in those cases the sugar-water and/or Ham tests
can be normal. It seems to work quite well. LFA-3 antibody is available
from AMAC; DAF and MIRL can be ordered from Research Diagnostics in New
Jersey - all have been of good quality in our hands. If you want to send
the sample to us we would be happy to run it - just drop me a separate note
directly for shipping info, etc.
-Brian
>Hello All;
>
> We have a patient here who we suspect has paroxysmal Nocturnal
>Hemoglobinuria (PNH), This disease represent a defficenty of numerous
>leukocyte antigens. The PIG protein or CD59 seems to be the critical
>lacking protein in these patients. While red cells are the ones most
>effected by the complement, white cells also lack this protein, and thus
>we can measure this by a simple flow procedure. My questions are two fold;
>
>1. does any one out there do CD59 staining, and would you like to tell me
>how I can SEND this sample to you. I really do not want to get into this
>bag of worms.
>
>2. Since no one probally does this assay in real life, where can I find CD
>59. I searched all my Catalogues, and no one seems to have it. Any
>pointers would wonderful.
>
>well thanks for all the help. take care -- rr
>
>rainer@pandoras-box.bgsm.wfu.edu
>
>
>
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