Enough of GFP?

RICHARD L. DARLEY (WHGRLD@cardiff.ac.uk)
Wed, 7 Jun 1995 16:50:14 GMT

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Has anyone any experience of measuring GFP expression by flow from
stably-integrated single copy vector in rapidly-dividing cells (DT
~20hrs).

I have seen no evidence that this is possible - but I'm sure someone
has tried by now. My particular interest in in stably infecting
human CD34+ cells with a retroviral vector bearing GFP as a marker.

I'd also be interested in any more news about artificial GFP mutants
at SER65 which are supposed to be up to 6x brighter than wt (Nature
373, 665).

Richard Darley
Dept Haematology
UWCM
Cardiff, UK

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu