Long term use of Fluo-3
(no name) ((no email))
Thu, 01 Jun 1995 16:33:34 -0400 (EDT)
Raymond Chang posted a question on long term (90 min) use of Fluo-3. The answer
is (as usual) that it depends to a very large extent on the cells used. In a
lymphoid cell line (CEM-SS) I found that there was good initial labeling but
that by 90 minutes there was overlap between labeled and unlabeled cells. Some
cell types have a Large Anion Transporter that will export all Fluo-3 in ~15
minutes, this transporter can be mostly blocked by probenicid.
I have never attempted to calibrate Fluo-3 levels in terms of uM or nM Ca++, I
seem to remember from old discussions (feel free to help me out here) that the
results were not good enough to be trusted.
Good luck, hope this helps.
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
James L. Weaver Email: WEAVER@FDACD.CDER.FDA.GOV
Molecular Pharmacology Phone: 301-594-5879
Division of Research & Testing FAX: 301-594-3037
ORR, CDER, Food & Drug Admin. Voice: 'Hey you'
Laurel, MD
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
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