Response to CD34 and apopto
Edward Srour (edward_srour@iucc.iupui.edu)
Tue, 30 May 1995 10:06:44 -0500
Steven Gore was asking for suggestions to label fixed cells with CD34. Try to
adopt the methodology we had used previously to study cell cycle status of
cultured CD34+ cells. You can find this in J. Immunol 148:815-20, 1992 or in
Exp Hematology 22:1264-72, 1994. Fixation of apoptotic cells with methanol
washes away the small size DNA fragments you are trying to label. Stick with
formaldehyde it works better.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
