Hoechst 33342 emission in apoptotic cells

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We are trying to detect apoptosis in Jurkat cells, with Ho33342.
We see a shift in Hoechst emission to longer wavelenghths in apoptotic cells
respect to non-apoptotic ones. Can anybody explain?

Thanks,

Jaume Comas
Unitat de Citometria de Flux
Serveis Cientifico-Tecnics.
Universitat de Barcelona, Spain.
E-mail jaume@giga.sct.ub.es

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu