Sorting G0 cells?

Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Tue, 14 May 1996 08:21:41 +1100

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Hi everyone,
Someone here wishes to sort cells from a human glioblastoma cell line,
separating out G0 and G1 fractions.
Collected cells must be intact, viable, etc. for further functional
study.

What markers can be used to separate G0 from G1 and the rest?
Any experience with handling glioblastoma cells?

Any ideas &/or suggestions appreciated, even if it is "don't bother"
(that could save me a lot...)

Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-565-6110 Fax: 61-2-565-6101

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