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> > Looking for a good permeableization reagent for TdT staining. Any
good > one's out there? Am planning to try Triton-X and CalTag's. Any
other > good procedures that could be used for bone marrows with 2 or 3
color > staining?
We published several years ago on formaldehyde/tx-100 for two color
staining (surface label before fixation). I have found that the
commercial products such as bd's don't always give adequate
permeabilization, although Mike Borowitz in heme/path here thinks its
terrific.
Steve Gore Johns Hopkins Oncology Center
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