RE: Re: Texas Red Laser Excitation

vanburen%flovax.dnet.wayne.edu@rocdec.roc.wayne.edu
Fri, 3 May 1996 20:36:18 -0400

Joseph Webster wrote: >569 should also excite PI, which may or may not
be useful. Is it too >low to excite APC?

It would appear so. Most references to APC either use 633nm (HeNe
laser), 647nm (Kr laser), or a Rhodamine 6G dye laser (tuned to
>590nm). The dye laser is the only laser that will simultaneously
excite TxRed and APC on a commercial sorter, however. At least until
the laser manufacturers can build an "orange" HeNe that puts out more
than 2mW power; they give you strange replies when you ask for 100mW at
594nm. :)

As for laser selection for a new sorter, I think everyone would have a
different opinion. If you can live without TxRed and APC
simultaneously, you can live without a dye laser (and pink floors). :)

/\/\/\_ Eric Van Buren, vanburen%flovax.dnet@rocdec.roc.wayne.edu
\ \ \ Analytical Cytometry, Karmanos Cancer Institute and
\_^_/ Immunology & Microbiology, Wayne State University, Detroit, MI


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