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> ... We're hoping to assess the proximity of two cell surface
molecules, using > fluorescence resonance energy transfer (FRET). We
have antibodies directly > conjugated to FITC and R.PE and would like
to use them to answer the question, > using FRET. ....
A possible mehod that allows you to measure fluorescence resonance
energy transfer was described in the following papers:
Szollosi et al., Cytomery, 5:210-216. 1984. Szollosi et al., Cytomery,
8:120-128. 1987, and Tron et al., Biophys.J. 45:939-946. 1984.
We have a free software to calculate FRET efficiency based on the above
method.
The software is a DOS application written in a language called FCAP
BASIC. It requires the FCB interpreter, which is also a free software.
Both required software files and a brief description of FCAP BASIC will
be available on the following World Wide Web site, within a few days:
http://www.wavefront.com/~soft-flow
The files can be downloaded from the site via internet. For help and
further information please contact the developers at fcap@fox.klte.hu.
The software can be changed easily to meet your special needs, too.
Best regards, Gyorgy Lustyik
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