Re: Texas Red Laser Excitation

• Messages sorted by: [ date ][ thread ][ subject ][ author ]
• Next message: BIGOS@Darwin.Stanford.EDU: "cellquest/powermacs/facsconvert"
• Previous message: Susan D. DEMAGGIO: "Confocal and Flow Users Group meeting final program"
• Maybe in reply to: Kristi R Harkins: "Texas Red Laser Excitation"
• Next in thread: Thomas Knapp: "Re: Texas Red Laser Excitation"

At 569nm Texas Red absorbs with about 50% of its maximum efficiency. We
have not tried this excitation, but my guess is that it would be quite
suboptimal for TR, with the low density antibodies being difficult to
separate from background. Moreover, 569nm is very near the excitation
peak for PE, so using this laser line for TR would preclude using PE
and any of its energy trasfer conjugates.

-Marty Bigos Stanford Shared FACS Facility

• Next message: BIGOS@Darwin.Stanford.EDU: "cellquest/powermacs/facsconvert"
• Previous message: Susan D. DEMAGGIO: "Confocal and Flow Users Group meeting final program"
• Maybe in reply to: Kristi R Harkins: "Texas Red Laser Excitation"
• Next in thread: Thomas Knapp: "Re: Texas Red Laser Excitation"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu