RE: Paraformaldehyde fixative

Libutti, Daniel A. (dal7@CIDDBD2.EM.CDC.GOV)
Tue, 30 Apr 96 15:45:00 EST

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I prefer paraformaldehyde prepared fresh weekly because it is free of
methanol and formic acid. Formaldehyde spontaneously produces formic acid in
solution which can denature proteins, besides it only takes 10 minutes every
week or two to make a batch and I know I am not introducing any further
unknowns.
Daniel
CDC, Atlanta
Methods Development
Immunology Section
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Subject: RE: Paraformaldehyde fixative
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Think about formalin. . . it's usable straight out of the bottle, and works
as well as paraformaldehyde for fixing cells. I don't know of any problems
with using it . . . my experience is paraformaldehyde and formalin give the
same results in immunofluorescence assays.

MAK.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu