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At 03:08 PM 29/04/96 -0400, you wrote:
>The "annoying reason" that Cellquest will not run old Lysis files (as you put
>it) is that Cellquest stores data in the FCS 2.0 file configuration and is
>designed to analyse those files. LYSIS II files are in FCS 1.0 file
Not Quite!
I believe that CellQuest COULD read other file formats, but deliberately
does not.
>configuration. File conversion is very rapid (about 10 seconds for the
>average 100-110K file) on my Quadra 650 running FACSCONVERT.
>I have had no difficulty converting Lysis II compatible files to Cellquest
>compatible ones.
But the converted files are NOT compatible with the rest of our systems!
Have you tried to analyse a converted file in PC-LYSYS or WinMDI?
Or even WinList if you can afford it?
For many good financial and historic reasons, all of our data acquisition
and routine analysis is done on HP and Windows computers, not Macs.
All our data storage and archiving is done in unix.
That means that extra copies have to be created (by conversion) and extra
storage space allocated for data to be analysed in CellQuest.
> The conversion is very rapid compared to the old FACSNET
>which took approximately 1 minute per 100k file to convert from PASCAL to DOS
>so one could run PC Lysis.
There is no need to convert in FACSnet!
We transfer files in BINARY mode with FACSnet to the unix server, and all
the analysis programs (except CellQuest!) read and analyse files directly
from the server disk.
In our system, FACSnet transfer takes about 1-2 seconds per standard 60Kb
file, and up to maybe 10 seconds for huge files.
For me, the bottom line is:
If you can afford to change ALL of your data acquisition, handling and
analysis over to CellQuest and Macintosh, then good luck and go ahead; most
of us cannot spent that much money all at once, no matter how much we and
BD would like us to.
Please don't take my diatribe personally, unless you are one of the company
decision makers who are continuing this mess of incompatibilities.
Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-565-6110 Fax: 61-2-565-6101
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