energy transfer
cheryl@immune.med.utoronto.ca
Mon, 29 Apr 1996 12:13:43 EDT
One of my users has a question concerning energy transfer between PE and FITC.
He does not subscribe to our mailing list, but I will turn the keyboard over to
him and you can either answer back to the list (I will pass responses along) or
you can reply to him directly at the E-mail address he will give you.
We're hoping to assess the proximity of two cell surface molecules, using
fluorescence resonance energy transfer (FRET). We have antibodies directly
conjugated to FITC and R.PE and would like to use them to answer the question,
using FRET.
There have been studies using the two as a donor and acceptor in the past via
the photobleaching method of FRET, but we would like to use the conventional
method of donor quenching. If you have any information on how to go about this
task, or know of any references where people have used these fluorophores with
this method I wolud be extremely grateful if you wolud contact me on my e-mail
address.
imad.nadra@utoronto.ca
Thank you very much for your help,
Imad Nadra.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
