Cell surface fluorescence after Fix & Perm

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We have experienced a dramatic decrease in PE fluorescence using CD4-PE
CD1-PE and CD45RA-PE after treating the cells with Fix and Perm from
Caltag when we stained human thymocytes and a whole blood preparation of
human PBL. Other antibodies conjugated to PE show only minor flurescence
decrease. In addition the same clone of CD4-PE maintaned its
fluorescence on PBL, but not on thynocytes.

Has anybody experienced similar problems? Can you suggest a solution on
how to prevent this? So far Caltag hasn't been able to provide anithing
but simpathy...

Thanks!!!!!

Ingrid Schmid
schmid@cyto.medsch.ucla.edu
UCLA Cellular Immunology and Cytometry

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