re:mpo by flow

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We have been using a method for permeabilising membranes which seems
to work very well. It works for all cytoplasmic and nuclear antigens
and is easy and quick to perform. Cells are fixed with
paraformaldehyde and permeabilized with Facs lysing solution
(Bectin Dickinson) and Tween 20. If you're interested in using the
method, the reference is j Clin Pathol,47,843-849 (1994) or
I'll be glad to send it to you.

Good Luck

Glenda

Glenda Davison
Department of Haematology
Groote Schuur Hospital
University of Cape Town
South Africa
email:gmd@samiot.uct.ac.za

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu