isotype controls -Reply
Karen Henell (henellk@ohsu.edu)
Tue, 26 Mar 1996 14:11:21 -0800
>>> Alice L. Givan <Alice.L.Givan@dartmouth.edu> 03/26/96 06:43am >>>
Just to second Mario Roederer's sound comments on isotype controls -- and
the complications of needing to match control concentration with stain
concentration (when we often don't know the concentration of either....).
There is, in addition, the added complication if using directly
conjugated antibodies. For the isotype control to be valid, it would
also have to match the staining antibody with respect to its F/P ratio.
And accurate determination of F/P ratios for antibodies used in flow
cytometry is a huge can of worms.....
Alice Givan
Englert Cell Analysis Laboratory Dartmouth Medical School
Lebanon, New Hampshire
USA
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
