lymphomas by flow -Reply
Karen Henell (henellk@ohsu.edu)
Sun, 24 Mar 1996 14:50:25 -0800
Karen Mann asked about media and transport effects on viability of lymph
node and lymphoma cells on 3/19/96.
We have good luck maintaining viability of T and B lymphocytes in normal
whole or even tissue-embedded lymph nodes using Hanks Balanced Salt
Solution and wet ice for the first 12-24 hours post excision. To improve
viability for longer storage or for more delicate cells our medium of
choice is RPMI 1640, again held at 4 degrees C.
Hope this helps.
Karen Henell
Histocompatibility Technologist
Laboratory of Immunogenetics and Transplantation
Oregon Health Sciences University
Portland, OR 97201 USA
henellk@ohsu.edu
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