RE; Isotype controls
dladouceur@isdtcp3.hwc.ca
Fri, 22 Mar 1996 11:10:54 -0500
2. Isotypic controls:
Steve,
>>>SO, I was wondering if all 5 isotypic controls needed could be
>>>batched together in one tube???
Thanks for your response to my question...
>If you put two different isotypes labeled with the same fluorochrome
>in the same tube and got some nonspecific binding, then to which
>isotype would you attribute the NSB signal?
But, provided the stained cells are clearly brighter than the
nsb, does it really matter which isotype you attribute the signal to?
(ie/ In other words, can one not say of the "combined" isotype ctl
tube: This would be the MAXIMUM nsb and everything above that is
positive).
Diane Ladouceur
<dladouceur@isdtcp3.hwc.ca>
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